Ku protein.

The Ku protein is an autoantigen that consists of 70- and 80-kDa polypeptides. It associates with double-stranded DNA at free ends. In the present study, we examined the ability of anti-Ku antibodies to immunoprecipitate various structures from extracts of HeLa cells prepared at different salt concentrations. Under physiological conditions ...

Ku protein. Things To Know About Ku protein.

When Ku protein was fractionated electrophoretically, transferred to nitrocellulose filter, and probed with 32P-labeled DNA, only the 70,000-dalton subunit exhibited DNA binding. Thus, the Ku protein appears to recognize selectively ends of double-stranded DNA molecules. Possible functions of the Ku autoantigen in eukaryotic cells are discussed.Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation. The Ku70NHEJ requires a protein complex consisting of the heterodimer of Ku70 and Ku80 (Ku complex) that recognizes DSB free ends and the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) (1, 5, 6) that recruits core NHEJ factors including Artemis, XRCC4, Ligase IV, and XLF on DSB break sites ().DNA-PKcs is a ∼470-kDa serine-threonine kinase belonging to the phosphoinositide 3-kinase-related ...Services Offered: Protein construct design, expression and purification for crystallization (gene-to-structure) Protein crystallization (only 12 mL of protein needed per 96-well screen) High throughput methods using robotics for crystallization and crystal imaging. Screening of protein ligand, inhibitor complexes and protein:protein complexes.

Ku protein from Methanobacterium formicicum and Methanocella paludicola, although derived from blast search using M. tuberculosis Ku, share a common ancestral node with Ku70 and Ku80. This shows that these two Ku proteins possibly arose from bacterial Ku before giving rise to Ku70 and Ku80 in eukaryotes. To validate this point, a search for ...The DNA-dependent protein kinase (DNA-PK) is a serine/threonine protein kinase composed of a large catalytic subunit (DNA-PKcs) and the Ku70/80 heterodimer. Over the past two decades, significant progress has been made in elucidating the role of DNA-PK in non-homologous end joining (NHEJ), the major pathway for repair of ionizing radiation-induced DNA double strand breaks in human cells and ...

6 Mar 2019 ... Al Awwaly, K.U. 2017. Protein Pangan Hasil Ternak dan Aplikasinya. Universitas. Brawijaya Press, Malang. Anonim. Bikin Es Krim. https ...Data for Ku cc -hDNA complex with a protein/DNA ratio of 1:1.2 were collected alone or in presence of pXLF or pAPLF peptides. Data were collected on the SWING beamline (SOLEIL Synchrotron) at a ...

Ku (red circle and red rectangle) is the most abundant DNA end binding protein in eukaryotic cells and can slide onto DNA ends that have diverse configurations . Once Ku is bound to the DNA end, it can improve the binding equilibrium of the nuclease, polymerases and ligase of NHEJ.The DNA-binding protein Ku plays a critical role in a variety of cellular processes, including the repair of double-stranded DNA breaks and V (D)J recombination. Paradoxically, while Ku is required for double-stranded break repair by non-homologous end-joining, in many organisms, Ku is also associated with telomeres.Core labs at KU benefit university investigators and industry partners alike. Each lab has experienced professionals and state-of-the-art technology to support you throughout your project. Working with core research labs provides multiple benefits: Full-time technicians to train you to use selected equipment.Alpha-fetoprotein (AFP) conversion to µg/L, ng/L, ng/dL, ng/100mL, ng%, ng/mL, IU/mL. Online conversion calculator for many types of measurement units in laboratory and medicine Alpha-fetoprotein (AFP), α1-fetoprotein, alpha-1-fetoprotein, alpha-fetoglobulin, alpha fetal protein

Here we address how the nature of a protein obstruction dictates how Ku interacts with a DNA end. Ku is unable to access the ends within an important intermediate in V(D)J recombination (a complex of RAG proteins bound to cleaved recombination targeting signals), but Ku readily displaces the linker histone, H1, from DNA.

INTRODUCTION. The Ku protein, a heterodimer consisting of Ku70 and Ku80 subunits, is a multifunctional complex playing critical roles in important cellular processes such as non-homologous end joining (NHEJ), V(D)J recombination, apoptosis, telomere maintenance and DNA replication ().The most well-studied function of Ku is its DNA-PKcs dependent role in NHEJ pathway, where it functions as a ...

Binding of Ku protein to DNA. Measurement of affinity for ends and demonstration of binding to nicks. J Biol Chem. 1993 Apr 5; 268 (10):7594-7601. [Google Scholar] Blunt T, Finnie NJ, Taccioli GE, Smith GC, Demengeot J, Gottlieb TM, Mizuta R, Varghese AJ, Alt FW, Jeggo PA, et al. Defective DNA-dependent protein kinase activity is linked to V ...A dimeric protein complex called KU initiates this process by binding to the broken DNA ends, then recruiting the DNA-PK catalytic subunit (DNA-PKcs) to form the active DNA-PK enzyme (Fig. 1a).Moreover, KU-55933 inhibits cancer cell proliferation by inducing G(1) cell cycle arrest. It does so through the downregulation of the synthesis of cyclin D1, a protein known to be elevated in a variety of tumors. In addition, KU-55933 treatment during serum starvation triggers apoptosis in these cancer cells.Ku proteins promote DNA binding and condensation of cyclic GMP-AMP synthase. Xinyue Tao 1 3 4 5. , Jiali Song 2 5. , Ying Song 2. , Yao Zhang 2. , Jing Yang …Recognition of DNA double-strand breaks during non-homologous end joining is carried out by the Ku70-Ku80 protein, a 150 kDa heterodimer that recruits the DNA repair kinase DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to the lesion. The atomic structure of a truncated Ku70-Ku80 was dete …

The data show that although Ku protein expression is relatively constant in the cytoplasmic and nuclear fractions in all cell lines, its association with chromatin was higher in the metastatic cells, while it was less in the hyperplastic cells and even less in the normal ones. By comparison, constant levels of Ku expression were observed in the ...The BJS_8160 was identified as a non-homologous end-joining protein Ku (Ku protein); these proteins are specialized in repairing DNA damages In S. meliloti Ku proteins have been identified in both free-living conditions and bacteroids , and related to stress conditions, as heat and nutrient starvation ; however, in S. meliloti, Ku protein was ...Ku is a complex of two proteins, Ku70 and Ku80, and functions as a heterodimer to bind DNA double-strand breaks (DSB) and activate DNA-dependent protein kinase.The acetylation of the DNA repair protein Ku in Mycobacterium smegmatis increases from exponential to stationary growth phase (35). In Bacillus subtilis, the rod shape-determining protein MreB is ...Services Offered: Protein construct design, expression and purification for crystallization (gene-to-structure) Protein crystallization (only 12 mL of protein needed per 96-well screen) High throughput methods using robotics for crystallization and crystal imaging. Screening of protein ligand, inhibitor complexes and protein:protein complexes. Autoantibodies to the DNA-binding Protein Antigen Ku 2275 urea gel, and detected by autoradiography with an intensifying screen at -70 "C. For analysis of protein, the antibody-coated beads were combined with 100 pl of 35S-labeled cell extract (equivalent to 1 X lo6 HeLa celis) and 400 pl of IPP buffer and incubated for 2 h at 4 "C. Following

The identification of DEB factor as Ku protein was first established by a useful adjunct to the EMSA, in which incubation of the binding reactions with anti-Ku antibodies produced a supershift of the original protein-DNA complex . Fig. 3. …

Chinese hamster ovary cell lines lacking XRCC7 showed 10-fold higher sensitivity to radiation and later the protein coded by the gene was identified as DNA-PK [16, 23, 24]. The critical finding that Ku protein is the regulatory component of DNA-PKcs unified both areas of research and gave a new dimension to the NHEJ field.The DNA end-binding protein Ku protein plays an important role in mammalian NHEJ. Owing to its ring-shaped structure, Ku threads onto exposed DNA termini (Fig. 1A).Following DNA binding by Ku, DNA-PK is assembled and the DNA Ligase IV complex is recruited to the site of the break (Fig. 1C).Highlighting the central role of Ku in NHEJ, deficiencies in Ku have been shown to result in defects in ...The GRAMM-X Protein Docking Web Server has been discontinued. The new GRAMM Docking Web Server can be found at ...Ku70 and Ku80 form a heterodimer called Ku protein, which is well known for its role in repairing DNA double‐strand breaks by non‐homologous end joining (NHEJ). 33 So, we are curious about the localization of Ku80, while Ku70 undergoes a translocation from the nucleus to the cytoplasm.Apr 15, 2021 · Ku is the first protein of the NHEJ repair complex to recognize and bind broken DNA ends, binding within 10 s of a DSB forming [ 180 ]. Ku threads onto both broken ends in a sequence-independent manner, with most models depicting a single heterodimer binding each end [ 1, 20 ]. The nuclear pore complex is the key regulator of transport between the cytoplasm and nucleus. Emerging evidence suggests it also regulates gene expression by influencing the internal architecture ...Introduction. Ku protein is an important component of the nonhomologous end‐joining (NHEJ) pathway of DNA double‐strand break repair, and it is ubiquitous among eukaryotes. 1-5 Some bacterial species encode a homolog of Ku, and bacterial Ku proteins function along with a dedicated ligase to repair DNA double‐strand breaks and protect against genome rearrangements (for review, see Ref. 6).

Two distinct and complementary pathways, non-homologous end joining (NHEJ) and homologous recombination (HR), are employed by mammalian cells to repair DNA DSBs. Each pathway is tightly controlled in response to increased DSBs. The Ku heterodimer has been shown to play a regulatory role in NHEJ repair. Ku80 ubiquitination contributes to the ...

Western-blot was used to determine the expression of Ku protein in total extract of CNE1 and CNE2 and semi-quantitative assay of protein expression was performed to estimate the optic density (OD) value of each band using automatic image analysis system. Results. Ku70/Ku80/DNA-PKcs primarily located in the nuclei.

The Ku protein is part of the DNA-dependent protein kinase (DNA-PK), which is required for the nonhomologous end-joining pathway of DNA double-strand break (DSB) repair (53, 59).When a break occurs, Ku binds avidly to the DNA ends, translocates inward, and recruits the 470-kDa DNA-PK catalytic subunit (DNA-PKcs) to form an active protein kinase complex (19, 26, 74).Here, we report that the Ku proteins (Ku80 and Ku70) directly interact with cGAS and positively regulate cGAS-mediated antiviral signaling. Mechanistically, we find that the interaction of the Ku proteins with cGAS significantly increases the DNA-binding affinity of cGAS and promotes cGAS condensation in the cytosol, thereby enhancing cGAS ...Ku protein is a heterodimer (Ku70 and Ku86) known to play an important role in V(D)J recombination, apoptosis, telomere fusion, and double-strand break repair. Its role in double-strand breaks is relevant to cancer therapy because lack of Ku86 causes one of the most radiation-responsive phenotypes (hamster cells, XRS5).The DNA-dependent protein kinase (DNA-PK) is a serine/threonine protein kinase composed of a large catalytic subunit (DNA-PKcs) and the Ku70/80 heterodimer. Over the past two decades, significant progress has been made in elucidating the role of DNA-PK in non-homologous end joining (NHEJ), the major pathway for repair of ionizing radiation-induced DNA double strand breaks in human cells and ...Here we address how the nature of a protein obstruction dictates how Ku interacts with a DNA end. Ku is unable to access the ends within an important intermediate in V(D)J recombination (a complex of RAG proteins bound to cleaved recombination targeting signals), but Ku readily displaces the linker histone, H1, from DNA.Ku is an abundant, highly conserved DNA binding protein found in both prokaryotes and eukaryotes that plays essential roles in the maintenance of genome integrity. In eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end binding factor in the “classical” non-homologous end joining (C-NHEJ) pathway ...Human protein Ku usually functions in the cell as a complex of two subunits, Ku70 and Ku80.The Ku heterodimer plays a key role in the non-homologous end joining DNA repair pathway by specifically recognizing the DNA ends at the site of the lesion. The binding of the Ku heterodimer to DNA has been well-studied, and its interactions with RNA have been also described.Although Ku80 requires Ku70 for binding to DNA, Ku70 may display some weak DNA-binding activity on its own (Chou et al., 1992; Griffith et al., 1992).Consistent with its role as a detector of DSBs, Ku binds linear DNA significantly better than closed supercoiled DNA (Blier et al., 1993).Similar to many other proteins involved in DNA metabolism (Hingorani and O'Donnell, 2000), Ku forms a toroid ...Ku protein consists of two subunits of approximately 70 and 86 kDa [22], [24]. Further characterization indicated that Ku protein binds primarily to double-stranded DNA ends (with either cohesive or blunt ends) and other structural discontinuities in DNA, such as nicks, gaps, and hairpins [1], [7], [23], [26].The Ku protein is a complex of two subunits, Ku70 and Ku80, and it plays a role in multiple nuclear processes, e.g., nonhomologous DNA-end-joining (NHEJ), chromosome maintenance, and transcription regulation. On the other hand, several studies have reported a cytoplasmic or cell surface localization of Ku in various cell types. The mechanism ...

About this page Nonhomologous End Joining in Bacteria E.J. Bartlett, A.J. Doherty, in Encyclopedia of Biological Chemistry (Second Edition), 2013 Bacterial Ku All Ku proteins form dimeric ring-like structures, capable of sliding over DNA ends. Ku does not bind to specific genetic sequences, nor make extensive contacts with the phosphate backbone.Ku protein (2 nM) binding red fluorescence aggregates in the mitochondria of healthy to non-radiolabeled 7SL RNA (8, 16 and 32 nM) and to hybrid 7SL-SC4 RNA (8, 16 and 32 nM) is shown in lanes 9-11 and 12-14, respectively. cells, and exists as green fluorescence monomers following a collapse of mitochondrial potential in dying cells. ...The Ku protein binds to DNA ends and other types of discontinuity in double-stranded DNA. It is a tightly associated heterodimer of ∼70 kDa and ∼80 kDa subunits that together with the ∼470 kDa catalytic subunit, DNA-PKcs, form the DNA-dependent protein kinase. This enzyme is involved in repairing DNA double-strand breaks (DSBs) caused ...Ku target antigen is a subunit heterodimer (70-80 kDa) associated with DNA-dependant protein kinase (350 kDa); on this dot, antigen coated is a human recombinant product obtained with baculovirus expression system. The secondary antibody is an alkaline phosphatase-conjugated goat anti-human IgG, and the substrate contains NBT/BCIP.Instagram:https://instagram. 2023 ktm 450 xcf w reviewcraigslist weatherford tx for salecollage concertrealistic weapon play gta 5 The DNA end-binding activity of Ku may also be involved in protection of chromosomal ends from nucleases, as yeast deficient in Ku70 or Ku80 display marked telomere attrition [1, 2]. Ku protein homologues have been identified in vertebrates, insects, Caenorhabditis elegans and yeast but not, to date, in prokaryotes. It is important to ...One of the advantages of PIFE is its dependence on the physical proximity of the protein and fluorescent dye, allowing for the spatial mapping of protein-DNA interactions. Although Ku is known ... scp 3812 powersandre ware twitter The inability to rescue activity with purified Ku suggests either that the role of Ku is indirect or that the endogenous Ku differs in some way from the exogenous protein. Interestingly, a different CHO cell mutant that lacks the DNA-dependent protein kinase catalytic subunit, a protein in the same repair pathway as Ku, also exhibits a decrease ... uk ku basketball Ku protein, together with Rap1p, a telomeric DNA (TG 1-3 repeat)-binding protein, is also required to initiate transcriptional silencing, or telomere-position effect (TPE). Here, we provide evidence for a direct role of Ku in TPE, which is most likely to be in either the recruitment or activation of Sir4 protein at the telomere. Surprisingly ...The Artemis protein and the Xrcc4/DNA ligase IV heterodimer are then recruited, with Artemis involved in the maturation of the DSB ends and the Xrcc4/DNA ligase IV complex catalysing the re-sealing of the ends [12], [13]. The KU heterodimer is composed of 70–86 kDa subunits, respectively called Ku70 and Ku80 (or Ku86) [14].